| Gene | Sema4g |
| Chromosome | 19 |
| Deletion Start | 44,992,518 |
| Deletion End | 45,002,085 |
| Deletion Size | 9,568 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 44,992,535 | 44,992,557 | CCTCCTCTTCAGCTTTCTCACAG |
| TUP | 44,992,558 | 44,992,580 | TAACAGCAGTGCCTGGACCCTCA |
| TUR | 44,992,586 | 44,992,606 | TCTAGCTCTCGGGATGGTCTC |
| TDF | 45,001,516 | 45,001,540 | CCATTGCTATACTTGGTGGCCTCTG |
| TDP | 45,001,547 | 45,001,573 | CTTGGCATCTTCCCTCCTCTATGTGGC |
| TDR | 45,001,580 | 45,001,599 | GACCTCGTCTGCCTCCCTTC |
PCR Primers
| Primer | Sequence |
| SU | AACCCTACAGTCCTCCTGGG |
| SD | GTGCACATGTGAATGGAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15109A-F7 | VGB6 | C57BL/6NTac | |
| 15109A-A9 | VGB6 | C57BL/6NTac | |
