Gene | Wnt3a |
Chromosome | 11 |
Deletion Start | 59,275,374 |
Deletion End | 59,249,532 |
Deletion Size | 25,843 |
Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
Primer | Start | End | Sequence |
TUF | 59,275,147 | 59,275,167 | GCCATCTTTGGCCCTGTTCTG |
TUP | 59,275,107 | 59,275,136 | CCCTGGGTGGTTAGGGTCATCTCTATATGG |
TUR | 59,275,071 | 59,275,090 | GCCCAGCCAGGTAGAGTTTG |
TDF | 59,250,343 | 59,250,363 | GTACTCCGTCCTAGACACTCC |
TDP | 59,250,315 | 59,250,342 | TGCAAAGAGAATTAGCTAGGCCCACAGG |
TDR | 59,250,275 | 59,250,293 | GCCAGATCCAAGCCCTCAG |
PCR Primers
Primer | Sequence |
SU | TCCTCACATGTTTTTTTAGAGG |
SD | GATAGAGCAGTGCCTCTGGG |
LacInR | TTGACTGTAGCGGCTGATGTTG |
LacInf | GGTAAACTGGCTCGGATTAGGG |
NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
NeoInF | TTCGGCTATGACTGGGCACAACAG |
LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
Forward Primer | Reverse Primer | Length | WT | HET | KO |
SU | LacZRev | bp | - | + | + |
TUF | TUR | 97 bp | + | + | - |
TDF | TDR | 89 bp | + | + | - |
NeoFwd | SD | bp | - | + | + |
LacInf | LacInR | 210 bp | - | + | + |
NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
15110A-H10 | VGB6 | C57BL/6NTac | |
15110B-E6 | VGB6 | C57BL/6NTac | |
15110B-G6 | VGB6 | C57BL/6NTac | |
15110C-F9 | VGB6 | C57BL/6NTac | |