| Gene | Wnt3a |
| Chromosome | 11 |
| Deletion Start | 59,275,374 |
| Deletion End | 59,249,532 |
| Deletion Size | 25,843 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 59,275,147 | 59,275,167 | GCCATCTTTGGCCCTGTTCTG |
| TUP | 59,275,107 | 59,275,136 | CCCTGGGTGGTTAGGGTCATCTCTATATGG |
| TUR | 59,275,071 | 59,275,090 | GCCCAGCCAGGTAGAGTTTG |
| TDF | 59,250,343 | 59,250,363 | GTACTCCGTCCTAGACACTCC |
| TDP | 59,250,315 | 59,250,342 | TGCAAAGAGAATTAGCTAGGCCCACAGG |
| TDR | 59,250,275 | 59,250,293 | GCCAGATCCAAGCCCTCAG |
PCR Primers
| Primer | Sequence |
| SU | TCCTCACATGTTTTTTTAGAGG |
| SD | GATAGAGCAGTGCCTCTGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15110A-H10 | VGB6 | C57BL/6NTac | |
| 15110B-E6 | VGB6 | C57BL/6NTac | |
| 15110B-G6 | VGB6 | C57BL/6NTac | |
| 15110C-F9 | VGB6 | C57BL/6NTac | |
