| Gene | Thbs1 |
| Chromosome | 2 |
| Deletion Start | 118,112,646 |
| Deletion End | 118,125,033 |
| Deletion Size | 12,388 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 118,113,306 | 118,113,325 | CAGGGAAGCAACAAGTGGTG |
| TUP | 118,113,328 | 118,113,351 | AGTGGAGGAAGCTCTCCTGGCCAC |
| TUR | 118,113,380 | 118,113,398 | GAGCCCGGTCCTCTTGAAC |
| TDF | 118,124,681 | 118,124,699 | TTCACGGGATGGCTTACAC |
| TDP | 118,124,702 | 118,124,727 | TGAGATCGGACCTACCGCAGAGTAAC |
| TDR | 118,124,747 | 118,124,768 | TGTGCTTGCAGACATCTGACTC |
PCR Primers
| Primer | Sequence |
| SU | CTGGTTCTCTTCAGCCTTCG |
| SD | AATGCTTCTGTGTGTGCTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15112A-A5 | VGB6 | C57BL/6NTac | |
| 15112A-G12 | VGB6 | C57BL/6NTac | |
