| Gene | Fzd5 |
| Chromosome | 1 |
| Deletion Start | 64,736,597 |
| Deletion End | 64,734,788 |
| Deletion Size | 1,810 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 64,736,346 | 64,736,364 | ACCGGACCTGCGCTTCTTC |
| TUP | 64,736,319 | 64,736,344 | TGTGCTCTATGTACACGCCCATCTGT |
| TUR | 64,736,288 | 64,736,306 | ACGGTGGTAGCGGCTTGTG |
| TDF | 64,735,527 | 64,735,546 | CCATCGCAGGTTATGCACAG |
| TDP | 64,735,505 | 64,735,526 | TACTTCCACCTTGCTGCCTGGC |
| TDR | 64,735,475 | 64,735,493 | GCGCCGTAATGGACTTGAC |
PCR Primers
| Primer | Sequence |
| SU | CTTGAGATTGGAGCTGGTTTG |
| SD | TCATTACAATGCCAATTTGAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15113A-F2 | VGB6 | C57BL/6NTac | |
| 15113B-C9 | VGB6 | C57BL/6NTac | |
| 15113B-F1 | VGB6 | C57BL/6NTac | |
| 15113C-B12 | VGB6 | C57BL/6NTac | |
