| Gene | Sema4a |
| Chromosome | 3 |
| Deletion Start | 88,454,814 |
| Deletion End | 88,436,575 |
| Deletion Size | 18,240 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 88,454,739 | 88,454,757 | CTCTTCCTGCTGCCATCAC |
| TUP | 88,454,717 | 88,454,738 | TGCCACCTGCTTCTGGGACTGG |
| TUR | 88,454,655 | 88,454,674 | ACCTCCCAGGTACTAGACAC |
| TDF | 88,437,382 | 88,437,401 | GAAGACAGATGGGCATGGTG |
| TDP | 88,437,356 | 88,437,380 | TCCATAGCAGGAATCCCAGACACCA |
| TDR | 88,437,303 | 88,437,321 | GTGGGAGAGCCAGCCTTAC |
PCR Primers
| Primer | Sequence |
| SU | TAAGGTGTCACAGCACAGGC |
| SD | GAGACTCTTGGGAAGGAGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 103 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15115A-A2 | VGB6 | C57BL/6NTac | |
| 15115A-D8 | VGB6 | C57BL/6NTac | |
| 15115A-E2 | VGB6 | C57BL/6NTac | |
| 15115A-H2 | VGB6 | C57BL/6NTac | |
| 15115A-A12 | VGB6 | C57BL/6NTac | |
