| Gene | Sema5a |
| Chromosome | 15 |
| Deletion Start | 32,646,486 |
| Deletion End | 32,689,412 |
| Deletion Size | 42,927 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | CCTGTCTTCATTTGGGTGCTG |
| TUP | | | AGGCCTCCCAGCCATTCAGGTCAG |
| TUR | | | AATGTTTAGTGCAGTGCTGATGTC |
| TDF | | | TCACCGTGAATCTCCCTTTCTC |
| TDP | | | CACCCTCTGTGCCACAGTGCCT |
| TDR | | | GCCTCCAGCCTAGCATCTTATG |
PCR Primers
| Primer | Sequence |
| SU | CGCTGAAACATCACCTAGTTTC |
| SD | CTGGACTGACCTCTTTCATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15117B-D1 | VGB6 | C57BL/6NTac | |
| 15117B-D2 | VGB6 | C57BL/6NTac | |
