| Gene | Vipr1 |
| Chromosome | 9 |
| Deletion Start | 121,642,852 |
| Deletion End | 121,669,612 |
| Deletion Size | 26,761 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 121,643,660 | 121,643,679 | CCAGCCACACTTGGACAGAG |
| TUP | 121,643,681 | 121,643,707 | TGATCTTCCCTTCCCTTGCTCTAGGTC |
| TUR | 121,643,739 | 121,643,762 | TGTTCCAGACCAGAATCCACTAAG |
| TDF | 121,668,732 | 121,668,751 | TTTGCCTCACCTCCTCTTCC |
| TDP | 121,668,762 | 121,668,785 | TTTGTGGTGGCCATCCTCTACTGC |
| TDR | 121,668,791 | 121,668,809 | GGCACTCACCTCGCCATTG |
PCR Primers
| Primer | Sequence |
| SU | TGTGGCCCTTCTAGTCCAAG |
| SD | GAGCTGGCAGATAAACGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 103 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15119A-G3 | VGB6 | C57BL/6NTac | |
| 15119E-A7 | VGB6 | C57BL/6NTac | |
