| Gene | Trpv3 |
| Chromosome | 11 |
| Deletion Start | 73,269,665 |
| Deletion End | 73,297,256 |
| Deletion Size | 27,592 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 73,270,002 | 73,270,025 | TGAAGCAAGAACATTCAGGAAGAC |
| TUP | 73,270,027 | 73,270,055 | CTTGGAGGGATAAGAGTTGCTGAAGCCAT |
| TUR | 73,270,070 | 73,270,093 | CCTAGAATAGCACCCACCCTTTAC |
| TDF | 73,296,504 | 73,296,524 | TGCAAAGTAGCAGATGAGGAC |
| TDP | 73,296,526 | 73,296,548 | TCCGGCTGTGTCTGCGGTAACAG |
| TDR | 73,296,557 | 73,296,578 | TCAAAGAATACTGCCGCACAAC |
PCR Primers
| Primer | Sequence |
| SU | TATGGCAGGAGATGGTGATG |
| SD | AGGTCCTCTACACACGCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15123A-H9 | VGB6 | C57BL/6NTac | |
| 15123A-G1 | VGB6 | C57BL/6NTac | |
