| Gene | Emid1 |
| Chromosome | 11 |
| Deletion Start | 5,152,219 |
| Deletion End | 5,128,875 |
| Deletion Size | 23,345 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 5,151,324 | 5,151,344 | TGCCTTCAAGACTGGAGGATG |
| TUP | 5,151,294 | 5,151,322 | AGGGAAACTTGTCCCCACTCAATAGAGGG |
| TUR | 5,151,263 | 5,151,282 | ACCCACCACGAGCACATTAG |
| TDF | 5,129,106 | 5,129,125 | CCACCCTGTCCTGCCTACTG |
| TDP | 5,129,081 | 5,129,105 | CAGCACAAGGCTGAGCCTCTGTCTT |
| TDR | 5,129,060 | 5,129,080 | GCCTGGGATTCCCTGAACAAC |
PCR Primers
| Primer | Sequence |
| SU | TTTCTCCTCCGTAACATGGG |
| SD | ATACAGAGTCCAAGAGGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 66 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15125A-E12 | VGB6 | C57BL/6NTac | |
| 15125A-C7 | VGB6 | C57BL/6NTac | |
