| Gene | Fgf22 |
| Chromosome | 10 |
| Deletion Start | 79,755,122 |
| Deletion End | 79,757,008 |
| Deletion Size | 1,887 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 79,755,733 | 79,755,753 | CCCATCCCAGTGGCTTTCATC |
| TUP | 79,755,754 | 79,755,777 | TGGGTTCCAAGGCCCTCATTAGGC |
| TUR | 79,755,784 | 79,755,803 | CCCTGTCATAGCCCTTGTCC |
| TDF | 79,756,458 | 79,756,476 | GCCAGCCTGTGCTCCTTAG |
| TDP | 79,756,477 | 79,756,498 | TCTCCAGGCTCCATGCCAGCTG |
| TDR | 79,756,513 | 79,756,532 | TCTTCAGTTCCCATCACCAC |
PCR Primers
| Primer | Sequence |
| SU | ATCGAGCATACCTCATTGCC |
| SD | GCGAACATGTCTCTGTCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15130A-A3 | VGB6 | C57BL/6NTac | |
| 15130A-A9 | VGB6 | C57BL/6NTac | |
| 15130A-B2 | VGB6 | C57BL/6NTac | |
| 15130A-B8 | VGB6 | C57BL/6NTac | |
| 15130A-C11 | VGB6 | C57BL/6NTac | |
| 15130A-C2 | VGB6 | C57BL/6NTac | |
