| Gene | Il22ra2 |
| Chromosome | 10 |
| Deletion Start | 19,622,043 |
| Deletion End | 19,632,920 |
| Deletion Size | 10,878 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 19,622,056 | 19,622,079 | GCCTTCTAGGTCTCCTCATCATAC |
| TUP | 19,622,083 | 19,622,110 | TGAGCAGTGCAACAGGTAAGTGCTTAAC |
| TUR | 19,622,145 | 19,622,165 | CCAAGCCATTCTAACCCTGTC |
| TDF | 19,632,494 | 19,632,515 | CACACCTCCAGAGTAACTGACC |
| TDP | 19,632,519 | 19,632,547 | CAAGTCCATGGCATTTCCTTACTCAGAGC |
| TDR | 19,632,577 | 19,632,600 | GAAGGCTAAGGATGGATGGAACAG |
PCR Primers
| Primer | Sequence |
| SU | CCCTGAGGCTTTGCTATCAC |
| SD | TCTTATTTGAGGGTTAAACCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15133A-F8 | VGB6 | C57BL/6NTac | |
| 15133A-E2 | VGB6 | C57BL/6NTac | |
| 15133A-H2 | VGB6 | C57BL/6NTac | |
