| Gene | Ccl20 |
| Chromosome | 1 |
| Deletion Start | 83,116,827 |
| Deletion End | 83,118,788 |
| Deletion Size | 1,962 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 83,116,853 | 83,116,874 | CCTTGCTTTGGCATGGGTACTG |
| TUP | 83,116,879 | 83,116,900 | CTCACCTCTGCAGCCAGGCAGA |
| TUR | 83,116,934 | 83,116,954 | ATGGTCCAGAGGACAGAAGTC |
| TDF | 83,117,809 | 83,117,831 | CGACTGTTGCCTCTCGTACATAC |
| TDP | 83,117,834 | 83,117,861 | ACGCCTCTTCCTTCCAGAGCTATTGTGG |
| TDR | 83,117,868 | 83,117,890 | AGCTTCATCGGCCATCTGTCTTG |
PCR Primers
| Primer | Sequence |
| SU | TGAGCTGCATTTCTTCCTCC |
| SD | TATGACTCTATCAACAAGTACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15134A-D6 | VGB6 | C57BL/6NTac | |
| 15134A-E11 | VGB6 | C57BL/6NTac | |
| 15134A-F6 | VGB6 | C57BL/6NTac | |
| 15134A-H1 | VGB6 | C57BL/6NTac | |
