15136 Fli1 Status: ES cell colonies screened / QC no positives
| Gene | Fli1 |
| Chromosome | 9 |
| Deletion Start | 32,476,770 |
| Deletion End | 32,452,137 |
| Deletion Size | 24,634 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 32,476,544 | 32,476,562 | GGTGAGCCTGCCAGAGTTG |
| TUP | 32,476,518 | 32,476,542 | CCAGTCCTCATGAAGCAGGGAAGCC |
| TUR | 32,476,454 | 32,476,473 | CTGACCCATCTGCTGCTTTG |
| TDF | 32,452,819 | 32,452,838 | AAGCCATTCCCAGCCTGACC |
| TDP | 32,452,792 | 32,452,817 | TCAGTCAGTTCCCTTGGCCACATGTC |
| TDR | 32,452,763 | 32,452,786 | GCCTTTCTAAGCACAACTCTTCTC |
PCR Primers
| Primer | Sequence |
| SU | GCAACTCTGTGTTTAGCCAC |
| SD | AAAATAGTTTCCCGAGGAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 109 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
