| Gene | Nms |
| Chromosome | 1 |
| Deletion Start | 38,939,289 |
| Deletion End | 38,948,672 |
| Deletion Size | 9,384 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 38,939,441 | 38,939,461 | ACCTTCACATCTGTCCTCCTG |
| TUP | 38,939,464 | 38,939,489 | TAGAGGAGCGGCACTTGTGTCTAACC |
| TUR | 38,939,490 | 38,939,509 | CTCACCGTGTCCATCACTCC |
| TDF | 38,948,360 | 38,948,378 | GCTGCTGTGTCTCCACCTG |
| TDP | 38,948,379 | 38,948,404 | AGACGCCTGGAGGCTAGATCCTCTCA |
| TDR | 38,948,432 | 38,948,455 | GACCTAACACCCATTCCCTACCTC |
PCR Primers
| Primer | Sequence |
| SU | TACAATGCTTACACTCGGCG |
| SD | GGCTTCAGCAGTCCCACTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15137A-D1 | VGB6 | C57BL/6NTac | |
| 15137A-E4 | VGB6 | C57BL/6NTac | |
