| Gene | Srpx2 |
| Chromosome | X |
| Deletion Start | 133,910,653 |
| Deletion End | 133,931,487 |
| Deletion Size | 20,835 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 133,910,695 | 133,910,714 | CTCCTAATGCCCGCAGTGAC |
| TUP | 133,910,716 | 133,910,743 | CCAACATGGTACGCAGGTAAGTTCTGGG |
| TUR | 133,910,746 | 133,910,767 | CCCTAGAAGGAACGGACAACAG |
| TDF | 133,931,274 | 133,931,295 | TGCTCTGTCTAGGCAATTCCAG |
| TDP | 133,931,296 | 133,931,323 | CGCCTCACTCGTTCCTACTTCAACATGG |
| TDR | 133,931,332 | 133,931,352 | CCGGTCAATACCCTGCTTGTC |
PCR Primers
| Primer | Sequence |
| SU | ATATTTGCACTTGATGGCCC |
| SD | CTGAAAGGCTTTGGGAAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15138B-A9 | VGB6 | C57BL/6NTac | |
| 15138B-H7 | VGB6 | C57BL/6NTac | |
| 15138C-H2 | VGB6 | C57BL/6NTac | |
