| Gene | Il17b |
| Chromosome | 18 |
| Deletion Start | 61,687,987 |
| Deletion End | 61,692,464 |
| Deletion Size | 4,478 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 61,688,827 | 61,688,850 | CTGGAAATGGAGTCTCACAGAAAC |
| TUP | 61,688,851 | 61,688,873 | CATGTGCCAGCTTCATGCTGCGT |
| TUR | 61,688,875 | 61,688,894 | CAGAGTGGGCGAGAAGAAGG |
| TDF | 61,692,386 | 61,692,404 | CCAGCGTGTCGTCATGGAG |
| TDP | 61,692,407 | 61,692,430 | CATCGCTGTGGGTTGCACCTGCAT |
| TDR | 61,692,431 | 61,692,451 | GGTTGGTGGTTGGCTCAGAAG |
PCR Primers
| Primer | Sequence |
| SU | CAGACACACAGGGCATTGTC |
| SD | TTTCCTGAGGGATTCTCCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 66 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15140A-B8 | VGB6 | C57BL/6NTac | |
| 15140A-H1 | VGB6 | C57BL/6NTac | |
