| Gene | Slc13a2 |
| Chromosome | 11 |
| Deletion Start | 78,422,164 |
| Deletion End | 78,397,599 |
| Deletion Size | 24,566 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 78,421,902 | 78,421,919 | TTTCCGCAGGGCACTTTG |
| TUP | 78,421,875 | 78,421,900 | AGCCTGCTGGTTTGTGGTCAGAAAGC |
| TUR | 78,421,842 | 78,421,861 | TCATCTGTCGGCTGCATTTC |
| TDF | 78,397,841 | 78,397,858 | TAGGCCCGTGCAGGATTC |
| TDP | 78,397,810 | 78,397,839 | TGCTCAATATCATCGGAGTGCTGACTATCA |
| TDR | 78,397,749 | 78,397,768 | GCCCAGGTGGGAAATGTGTC |
PCR Primers
| Primer | Sequence |
| SU | AGCTCCAAGGGTCATGTGTC |
| SD | TGAACACCGGGAAACACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 110 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15141A-A3 | VGB6 | C57BL/6NTac | |
| 15141A-C5 | VGB6 | C57BL/6NTac | |
| 15141A-D1 | VGB6 | C57BL/6NTac | |
| 15141A-D10 | VGB6 | C57BL/6NTac | |
| 15141A-D11 | VGB6 | C57BL/6NTac | |
| 15141A-F10 | VGB6 | C57BL/6NTac | |
