| Gene | Chid1 |
| Chromosome | 7 |
| Deletion Start | 141,532,935 |
| Deletion End | 141,495,965 |
| Deletion Size | 36,971 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 141,532,229 | 141,532,250 | TTACTCCTTAGGCCCAACAGTC |
| TUP | 141,532,206 | 141,532,227 | CCATACTGCTGGGCAGGCTGCT |
| TUR | 141,532,149 | 141,532,168 | CCCAAGGAACAGAGCATCTC |
| TDF | 141,496,857 | 141,496,877 | ACAGTGGGACTTGGAGGTTTG |
| TDP | 141,496,827 | 141,496,855 | CCACCCAAGCTTACTGGCTGAACATAACA |
| TDR | 141,496,808 | 141,496,826 | GTGAGCCAGCAGTGCTTTG |
PCR Primers
| Primer | Sequence |
| SU | TTGCCCATCGTATGTTTGAG |
| SD | TAAGTCCAGCTTCTCTGGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15147A-A10 | VGB6 | C57BL/6NTac | |
| 15147A-A12 | VGB6 | C57BL/6NTac | |
| 15147A-D6 | VGB6 | C57BL/6NTac | |
| 15147A-H1 | VGB6 | C57BL/6NTac | |
| 15147A-D4 | VGB6 | C57BL/6NTac | |
| 15147A-E12 | VGB6 | C57BL/6NTac | |
