| Gene | Cul5 |
| Chromosome | 9 |
| Deletion Start | 53,667,058 |
| Deletion End | 53,642,522 |
| Deletion Size | 24,537 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 53,666,451 | 53,666,471 | TGTCCCTTGAGGAACTGTCTC |
| TUP | 53,666,421 | 53,666,450 | CCAATCCTGACCTCCCTTATAGGACATCGA |
| TUR | 53,666,325 | 53,666,346 | GGACTCTCCTGGCATTTACAAC |
| TDF | 53,643,358 | 53,643,375 | GCACCTTTGCCTGTCACC |
| TDP | 53,643,333 | 53,643,356 | TCACACCAGCCTGCTGCTTAGTGG |
| TDR | 53,643,277 | 53,643,298 | GCTGCCTACAAGAATGGAAGTG |
PCR Primers
| Primer | Sequence |
| SU | GGCCCTAAGCCGAGATAAAG |
| SD | ACCAGTTCCAGATATCTAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 147 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15150A-D10 | VGB6 | C57BL/6NTac | |
| 15150B-E5 | VGB6 | C57BL/6NTac | |
| 15150B-F10 | VGB6 | C57BL/6NTac | |
| 15150B-H7 | VGB6 | C57BL/6NTac | |
| 15150B-H12 | VGB6 | C57BL/6NTac | |
| 15150C-H1 | VGB6 | C57BL/6NTac | |
