| Gene | Cib2 |
| Chromosome | 9 |
| Deletion Start | 54,560,015 |
| Deletion End | 54,545,402 |
| Deletion Size | 14,614 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 54,559,442 | 54,559,462 | TCTGTAGGTATGCGAGGGAAC |
| TUP | 54,559,420 | 54,559,441 | CTTATCTCCGGGCTGGCCCAGA |
| TUR | 54,559,390 | 54,559,411 | ACATGGTGAGCTAAAGCAACTG |
| TDF | 54,546,111 | 54,546,131 | CTCAGCCCTCTTCTGACCATC |
| TDP | 54,546,084 | 54,546,110 | TCCTTCCATCCCTCCTAACCTCACAGA |
| TDR | 54,546,031 | 54,546,050 | GGGCCAGCGTCATCTCTAAG |
PCR Primers
| Primer | Sequence |
| SU | AGTGTCCAGTAGTCAGGCGG |
| SD | AAATCCAGCCTCACCTCTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 101 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15151A-B12 | VGB6 | C57BL/6NTac | |
| 15151A-C6 | VGB6 | C57BL/6NTac | |
| 15151A-F5 | VGB6 | C57BL/6NTac | |
| 15151A-F6 | VGB6 | C57BL/6NTac | |
