| Gene | Cog6 |
| Chromosome | 3 |
| Deletion Start | 53,017,195 |
| Deletion End | 52,983,029 |
| Deletion Size | 34,167 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 53,016,848 | 53,016,869 | TCTTATCAGAGCTGGCTGTCTC |
| TUP | 53,016,823 | 53,016,847 | CCCTGCAGCTTTGAGTAGCCAGGTA |
| TUR | 53,016,774 | 53,016,793 | TTACGGCTCCCACCTTACTG |
| TDF | 52,983,215 | 52,983,235 | TGCTGGGCTTCTGTTATCTTC |
| TDP | 52,983,187 | 52,983,214 | TGGTTGTGTTTGCTCATCCATAGGGAGC |
| TDR | 52,983,137 | 52,983,155 | CACTTCCGCGTAGGCTCTG |
PCR Primers
| Primer | Sequence |
| SU | GCATGCCAGCTACTGGTTTC |
| SD | CAATTAGAGGAAAGCAGCGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 96 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15162A-G8 | VGB6 | C57BL/6NTac | |
| 15162A-F2 | VGB6 | C57BL/6NTac | |
| 15162A-G5 | VGB6 | C57BL/6NTac | |
| 15162A-A7 | VGB6 | C57BL/6NTac | |
