| Gene | Cdh23 |
| Chromosome | 10 |
| Deletion Start | 60,657,330 |
| Deletion End | 60,650,095 |
| Deletion Size | 7,236 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 60,657,306 | 60,657,328 | GTACTCCCTGGTCACTTGCTATG |
| TUP | 60,657,281 | 60,657,304 | TGTGCTCTGGCTTCTCATGCTGGT |
| TUR | 60,657,230 | 60,657,251 | GCACTGGCTGGCATTATCAAAG |
| TDF | 60,650,302 | 60,650,320 | CCAGTGCTCAGCAGCTATC |
| TDP | 60,650,278 | 60,650,301 | TGTGGCCAACCTGACTGTCTTGGG |
| TDR | 60,650,216 | 60,650,240 | ACATATCCTACAGAGTGACACAGAG |
PCR Primers
| Primer | Sequence |
| SU | TGTTGGATCTGCGTTCAGAG |
| SD | CTTGGAGCAGAAGAGAAGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 105 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15164A-B8 | VGB6 | C57BL/6NTac | |
| 15164A-D4 | VGB6 | C57BL/6NTac | |
| 15164A-G5 | VGB6 | C57BL/6NTac | |
| 15164A-H1 | VGB6 | C57BL/6NTac | |
| 15164A-D11 | VGB6 | C57BL/6NTac | |
| 15164A-F12 | VGB6 | C57BL/6NTac | |
