15172   Metrnl     Status: ES cell colonies screened / QC positives

GeneMetrnl
Chromosome11
Deletion Start121,702,629
Deletion End121,716,525
Deletion Size13,897
Genome Build38
MGI IDMGI:2384806
NCBI Gene ID210029
BAC ID370p11
BAC LibraryBAC ES release 2
CassetteZen-Ub1-Hyg-avrII
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 11

Please be aware that these oligonucleotides have been validated only by Taqman assay.

Taqman primers not yet available.

PCR Primers

PrimerSequence
SU485SU
SD485SD
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
15172A-B12VGB6C57BL/6NTac
15172B-G10VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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