15176   Esm1     Status: ES cell clone microinjected

GeneEsm1
Chromosome13
Deletion Start113,209,852
Deletion End113,216,750
Deletion Size6,899
Genome Build38
MGI IDMGI:1918940
NCBI Gene ID71690
BAC ID243e2
BAC LibraryBAC ES release 2
CassetteZen-Ub1-Hyg-avrII
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 13

Please be aware that these oligonucleotides have been validated only by Taqman assay.

Taqman primers not yet available.

PCR Primers

PrimerSequence
SU542SU
SD542SD
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
15176A-E9VGB6C57BL/6NTac
15176A-E10VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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