| Gene | Il16 |
| Chromosome | 7 |
| Deletion Start | 83,722,593 |
| Deletion End | 83,722,289 |
| Deletion Size | 305 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 83,722,520 | 83,722,538 | CCCGTTCCCTGATCCTCTG |
| TUP | 83,722,495 | 83,722,516 | CGCCAAGACCAGTGATGACGGC |
| TUR | 83,722,442 | 83,722,461 | CCCTGGCACAGTGAAGTCTC |
| TDF | 83,722,374 | 83,722,394 | AGGCTGGGCTCAGCAATATCC |
| TDP | | | AGACCTCGCACTGGCC |
| TDR | 83,722,334 | 83,722,353 | GCTGCGAGTTGAGCAGAGTC |
PCR Primers
| Primer | Sequence |
| SU | GCCTTTCCCATGATTCTGAG |
| SD | TATGCCAAAGGATGCCTGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 61 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15184A-B7 | VGB6 | C57BL/6NTac | |
| 15184B-F1 | VGB6 | C57BL/6NTac | |
