| Gene | Reg3d |
| Chromosome | 6 |
| Deletion Start | 78,378,503 |
| Deletion End | 78,376,007 |
| Deletion Size | 2,497 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 78,378,462 | 78,378,481 | GCATGTCCTGGATGCTACTG |
| TUP | 78,378,435 | 78,378,461 | TGTTGCCTGATGTCCCTTTCTTGGGTA |
| TUR | 78,378,409 | 78,378,431 | GCTAGACACACAACAACTTTACC |
| TDF | 78,376,463 | 78,376,486 | TCTGACCCTCTGACCTTCTATAAC |
| TDP | 78,376,437 | 78,376,462 | TGGGAGATACCACCCTCCATGTCTGC |
| TDR | 78,376,405 | 78,376,427 | CCTGAGACAAAGCTGCACAGTAG |
PCR Primers
| Primer | Sequence |
| SU | AGGAGCTGGCACTTCTTCAC |
| SD | GGCAATGCCTTCATTTGTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15187A-A10 | VGB6 | C57BL/6NTac | |
| 15187A-G4 | VGB6 | C57BL/6NTac | |
