| Gene | Flt4 |
| Chromosome | 11 |
| Deletion Start | 49,651,159 |
| Deletion End | 49,624,665 |
| Deletion Size | 26,495 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 49,625,307 | 49,625,327 | GGGTTGTGCATGACTGTGAAG |
| TUP | 49,625,329 | 49,625,355 | CACAGAAGCTAGGCCCTACTGCAAGGT |
| TUR | 49,625,380 | 49,625,397 | TAGCTGCCCGTGTTGTTG |
| TDF | 49,650,798 | 49,650,818 | TCTGTTTGCGTCTGGTGATTC |
| TDP | 49,650,829 | 49,650,848 | CTGACCCAGGGCCACGCTCA |
| TDR | 49,650,864 | 49,650,884 | CTGCCAAGACACGGAGCATAG |
PCR Primers
| Primer | Sequence |
| SU | AGGATTGGACTCAGTCTGTG |
| SD | AGGAGAAGGAAGCAGGAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15191A-A4 | VGB6 | C57BL/6NTac | |
| 15191A-B4 | VGB6 | C57BL/6NTac | |
| 15191A-E5 | VGB6 | C57BL/6NTac | |
| 15191A-F7 | VGB6 | C57BL/6NTac | |
| 15191A-G3 | VGB6 | C57BL/6NTac | |
| 15191A-H11 | VGB6 | C57BL/6NTac | |
