| Gene | Tmem119 |
| Chromosome | 5 |
| Deletion Start | 113,795,735 |
| Deletion End | 113,794,858 |
| Deletion Size | 878 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 113,795,451 | 113,795,468 | ATGCTCATCGCGGTGGTG |
| TUP | 113,795,418 | 113,795,447 | TCGCTGACCTTCCTCATCATGTTCATAGTC |
| TUR | 113,795,379 | 113,795,396 | TGTGGCCTTGTGCTTCTG |
| TDF | 113,795,225 | 113,795,243 | CAGCTCCAGGCTGACATTC |
| TDP | 113,795,203 | 113,795,224 | TGGCTGCTACCCAGAACCTCCG |
| TDR | 113,795,145 | 113,795,164 | CCCACCCTTCACAGGCTTTG |
PCR Primers
| Primer | Sequence |
| SU | GATGCCTGGTTAGCCATGAG |
| SD | GGTGATGGGAAGATGCAAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15207A-G10 | VGB6 | C57BL/6NTac | |
| 15207A-E4 | VGB6 | C57BL/6NTac | |
| 15207A-G8 | VGB6 | C57BL/6NTac | |
