| Gene | Cd300lf |
| Chromosome | 11 |
| Deletion Start | 115,133,878 |
| Deletion End | 115,116,933 |
| Deletion Size | 16,946 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 115,133,838 | 115,133,860 | CCCTTTCTCTTCTGGATCACAGG |
| TUP | 115,133,815 | 115,133,837 | TGAGCGAGCTCAGGCAACCAGAG |
| TUR | 115,133,776 | 115,133,794 | GGGTGGTGGACAGATGACC |
| TDF | 115,117,474 | 115,117,493 | CACCGCCTTGGAAAGTGAAC |
| TDP | 115,117,449 | 115,117,473 | TCCATACCCATGTGGACACAGCTGA |
| TDR | 115,117,413 | 115,117,431 | TCTGAAGCCGCTTGCTCTG |
PCR Primers
| Primer | Sequence |
| SU | CTCTTCTGGCACCCTTCAAC |
| SD | CGTTGATACGATTTACACAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15209A-C3 | VGB6 | C57BL/6NTac | |
| 15209A-C4 | VGB6 | C57BL/6NTac | |
| 15209A-E10 | VGB6 | C57BL/6NTac | |
| 15209A-G4 | VGB6 | C57BL/6NTac | |
