| Gene | Bmp3 |
| Chromosome | 5 |
| Deletion Start | 98,879,931 |
| Deletion End | 98,855,116 |
| Deletion Size | 24,816 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 98,855,133 | 98,855,154 | GCTCTGTCTATGGCTAGGTTAC |
| TUP | 98,855,157 | 98,855,179 | CTGCCTGAACCTGGCACAGGGAC |
| TUR | 98,855,183 | 98,855,201 | GGGAGGTGCAAGTTTGGTC |
| TDF | 98,879,535 | 98,879,559 | TGTGAAGATTAAAGGCACCACCTAC |
| TDP | 98,879,564 | 98,879,587 | TTCCCGGACCCTAACAGGCGCTTT |
| TDR | 98,879,606 | 98,879,627 | TCAGCCCAAACTCATCTACCTC |
PCR Primers
| Primer | Sequence |
| SU | AACTCAGCTGGCTGCGAC |
| SD | AGTCACTGTGTTCTCTAACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15210A-A8 | VGB6 | C57BL/6NTac | |
| 15210A-F7 | VGB6 | C57BL/6NTac | |
