| Gene | Chrd |
| Chromosome | 16 |
| Deletion Start | 20,742,023 |
| Deletion End | 20,733,151 |
| Deletion Size | 8,873 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 20,733,908 | 20,733,926 | GGACGGGCCATGTAACTTG |
| TUP | 20,733,932 | 20,733,955 | AGCAGAATTGACTGTCTGCAGCCC |
| TUR | 20,733,992 | 20,734,011 | TGAGGCTGATGGCAGAGAAC |
| TDF | 20,741,514 | 20,741,532 | GAGCGGGACGATTGTTCAC |
| TDP | 20,741,533 | 20,741,554 | CGCCGCTATCCTGTGGCTCAGG |
| TDR | 20,741,589 | 20,741,611 | GGCTTCCTTCACTTACACCTTTG |
PCR Primers
| Primer | Sequence |
| SU | CGTTTGCTGAAGAGGAGGC |
| SD | ATCCCAATAAGCCAGTGGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 104 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15213A-A1 | VGB6 | C57BL/6NTac | |
| 15213A-F3 | VGB6 | C57BL/6NTac | |
| 15213B-G10 | VGB6 | C57BL/6NTac | |
| 15213B-F11 | VGB6 | C57BL/6NTac | |
| 15213B-A6 | VGB6 | C57BL/6NTac | |
| 15213B-E4 | VGB6 | C57BL/6NTac | |
