| Gene | Smn1 |
| Chromosome | 13 |
| Deletion Start | 100,137,468 |
| Deletion End | 100,124,897 |
| Deletion Size | 12,572 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 100,125,528 | 100,125,549 | GGTAAGTACGAGGGCAAATCAG |
| TUP | 100,125,553 | 100,125,579 | CAAGTTGGTGGACTGTGCATGTGATCA |
| TUR | 100,125,603 | 100,125,624 | CCCAGGCTAGACTCAACTTCAC |
| TDF | 100,137,380 | 100,137,400 | GGTTCAGCTCTGTCTCAGGAG |
| TDP | 100,137,407 | 100,137,429 | TGTCGGTGTCCCTGGTCGACAAG |
| TDR | 100,137,439 | 100,137,459 | GAGTCCACTGATGACGAGGAG |
PCR Primers
| Primer | Sequence |
| SU | TGCATCAAGAACCAGCAAAC |
| SD | GCACATTTGTGCTCAGTCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15224A-C4 | VGB6 | C57BL/6NTac | |
| 15224A-C8 | VGB6 | C57BL/6NTac | |
| 15224A-E1 | VGB6 | C57BL/6NTac | |
| 15224A-E4 | VGB6 | C57BL/6NTac | |
| 15224A-F2 | VGB6 | C57BL/6NTac | |
