| Gene | Tmem184b |
| Chromosome | 15 |
| Deletion Start | 79,378,652 |
| Deletion End | 79,362,587 |
| Deletion Size | 16,066 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 79,378,225 | 79,378,244 | GCTACGACCTGCTCAGAATC |
| TUP | 79,378,201 | 79,378,221 | TGGCCACGCACATATGCAGCC |
| TUR | 79,378,156 | 79,378,176 | CCTCAGGCTCCCAATAATGTC |
| TDF | 79,363,270 | 79,363,290 | GGGCACAGGTGGATGAGTTAG |
| TDP | 79,363,241 | 79,363,266 | CAAGTCAAGGTAGCGAGTTCCTGCCC |
| TDR | 79,363,181 | 79,363,199 | GGCTGCTGAACCAGGTCTC |
PCR Primers
| Primer | Sequence |
| SU | TTCTGTACCCTGCTTCCCAG |
| SD | AGGGTCCACAGAGCATTCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 110 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15229A-C7 | VGB6 | C57BL/6NTac | |
| 15229A-A7 | VGB6 | C57BL/6NTac | |
