| Gene | A530098C11Rik |
| Chromosome | 1 |
| Deletion Start | 44,017,264 |
| Deletion End | 44,009,637 |
| Deletion Size | 7,628 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 44,016,529 | 44,016,550 | GAGGTAACTTGCCCAGAGTGAG |
| TUP | 44,016,500 | 44,016,527 | TCTCTACTTCTACCGTGAGGGTTCAGGA |
| TUR | 44,016,467 | 44,016,486 | TGCTTGCTTCTGAGCCTGAG |
| TDF | 44,009,830 | 44,009,852 | TGTTGTCTACCACCACTACTTCC |
| TDP | 44,009,806 | 44,009,829 | TGGACAAGCTGCTCGCTACCATGG |
| TDR | 44,009,765 | 44,009,782 | GTTTGCCCACAGCACCAC |
PCR Primers
| Primer | Sequence |
| SU | TCCAAGCTGGGTTTCTTCAC |
| SD | GTGGCACACGGCTGTAATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15237A-H12 | VGB6 | C57BL/6NTac | |
| 15237A-C6 | VGB6 | C57BL/6NTac | |
| 15237A-H3 | VGB6 | C57BL/6NTac | |
| 15237A-G3 | VGB6 | C57BL/6NTac | |
