| Gene | Carf |
| Chromosome | 1 |
| Deletion Start | 60,132,029 |
| Deletion End | 60,108,129 |
| Deletion Size | 23,901 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 60,108,766 | 60,108,786 | AAGGGTCACGGCATTAGGAAG |
| TUP | 60,108,788 | 60,108,811 | TGGGAACCACTGGCTGAAGACAAC |
| TUR | 60,108,821 | 60,108,845 | CAACCATTTACACTGAGCTACATTC |
| TDF | 60,131,910 | 60,131,929 | CACAAGCAGCAAACACAGAC |
| TDP | 60,131,939 | 60,131,960 | TACTCGCCAGTCTCCAAGCCCA |
| TDR | 60,131,981 | 60,132,004 | GGGACATACTGAGACTTCCACATC |
PCR Primers
| Primer | Sequence |
| SU | GACAGAATGAGAGAACTACG |
| SD | GTGATATTGTCTATAAGTTCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15239A-C1 | VGB6 | C57BL/6NTac | |
| 15239A-G6 | VGB6 | C57BL/6NTac | |
