15244 Pax3 Status: ES cell colonies screened / QC no positives
| Gene | Pax3 |
| Chromosome | 1 |
| Deletion Start | 78,196,746 |
| Deletion End | 78,192,448 |
| Deletion Size | 4,299 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 78,196,442 | 78,196,464 | TGCTAAGCTCACTACAAGCTCTC |
| TUP | 78,196,418 | 78,196,436 | TTGCCTGGCCTGCTCCTGC |
| TUR | 78,196,380 | 78,196,400 | CGGCAGCAATTCCGAAACAAC |
| TDF | 78,192,704 | 78,192,725 | CGGATGCTCACAGATGCAGAAC |
| TDP | 78,192,680 | 78,192,703 | ACAGCACTGAGCCCTGCTCAAGGG |
| TDR | 78,192,648 | 78,192,669 | AGGCCCAGCTAACGTACTCAAC |
PCR Primers
| Primer | Sequence |
| SU | AGACTTGGGACGTGTTCCTG |
| SD | GACTTCTCAACTCTAAGGAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
