| Gene | Lrp4 |
| Chromosome | 2 |
| Deletion Start | 91,498,443 |
| Deletion End | 91,471,143 |
| Deletion Size | 27,301 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 91,471,223 | 91,471,244 | GCAGTGTGATGGAGACAATGAC |
| TUP | 91,471,250 | 91,471,275 | AGACCACAGCGATGAGGATGGCTGTA |
| TUR | 91,471,336 | 91,471,354 | TGGACACAAGGCAGGCAAG |
| TDF | 91,497,996 | 91,498,015 | GTTGGTAGGGTGACCTCTTC |
| TDP | 91,498,021 | 91,498,046 | ACCTTGGTTAGGTAGGGCCACATGGC |
| TDR | 91,498,088 | 91,498,105 | GGGTCCTAGCCGGAAATC |
PCR Primers
| Primer | Sequence |
| SU | TCTTGTTCTGAGCACAACATC |
| SD | AATCTCTCACTTTTAGCCATTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 132 bp | + | + | - |
| TDF | TDR | 110 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15248A-G7 | VGB6 | C57BL/6NTac | |
