| Gene | Lrp6 |
| Chromosome | 6 |
| Deletion Start | 134,542,040 |
| Deletion End | 134,513,229 |
| Deletion Size | 28,812 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 134,541,944 | 134,541,965 | GTAGTTGGAGGCTTGGAGGATG |
| TUP | 134,541,908 | 134,541,934 | TGGACTTTGTGTTTGGTCATGGCTTGA |
| TUR | 134,541,871 | 134,541,893 | CGTTTAATGGCTTCTTCGCTGAC |
| TDF | 134,513,672 | 134,513,689 | GGGAGGCTGACAGCAATG |
| TDP | 134,513,641 | 134,513,669 | AAGAGATTTGGGCTTCTGTGTCTTGTGAC |
| TDR | 134,513,612 | 134,513,631 | GGTCCCTGCCTTGGAAGTAG |
PCR Primers
| Primer | Sequence |
| SU | TCTCAATAAGAAAAGACAGACAG |
| SD | AACAGCAGATGAGCTGTCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15249B-F11 | VGB6 | C57BL/6NTac | |
