| Gene | Dcst2 |
| Chromosome | 3 |
| Deletion Start | 89,365,403 |
| Deletion End | 89,373,663 |
| Deletion Size | 8,261 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 89,365,967 | 89,365,988 | GTTGCTTTCTGCTGGAATTGTG |
| TUP | 89,365,989 | 89,366,012 | TCCTACCCTAAGAGTGGCTGGACC |
| TUR | 89,366,030 | 89,366,050 | GGGAAGAGGATTTAGCTGGAC |
| TDF | 89,373,157 | 89,373,177 | CAAACACTAGGGAGCACAAGG |
| TDP | 89,373,187 | 89,373,211 | TTAGCTCAAGCCTCCTTCCCTCCTG |
| TDR | 89,373,223 | 89,373,245 | GAAACAGGTAGGGCAGTAGAGAC |
PCR Primers
| Primer | Sequence |
| SU | TGTGTCCTTCAACTCCTCAG |
| SD | TCTCTCCTTTTCAGTGAAGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15251A-D5 | VGB6 | C57BL/6NTac | |
| 15251A-D10 | VGB6 | C57BL/6NTac | |
| 15251A-D8 | VGB6 | C57BL/6NTac | |
| 15251A-D12 | VGB6 | C57BL/6NTac | |
| 15251A-G9 | VGB6 | C57BL/6NTac | |
| 15251A-C6 | VGB6 | C57BL/6NTac | |
