| Gene | Lrfn3 |
| Chromosome | 7 |
| Deletion Start | 30,360,795 |
| Deletion End | 30,355,578 |
| Deletion Size | 5,218 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 30,360,628 | 30,360,649 | TCTATTCGTACCGCCATCACTG |
| TUP | 30,360,606 | 30,360,626 | ATCGCCGCGCTGCAGAACTGC |
| TUR | 30,360,580 | 30,360,597 | TGCCGCGATGAAGTTGTC |
| TDF | 30,356,157 | 30,356,175 | GGAGGCACAGGGCAGATAG |
| TDP | 30,356,130 | 30,356,155 | TCTTGGGTATTGGGTGACCACAGTCC |
| TDR | 30,356,102 | 30,356,123 | TCCTGAGGGAGGGTGATAGATG |
PCR Primers
| Primer | Sequence |
| SU | TCTCTGTAGCTCGTCTAGAC |
| SD | CACAGCTCGGTCCCTAGAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15252A-A12 | VGB6 | C57BL/6NTac | |
| 15252A-E7 | VGB6 | C57BL/6NTac | |
| 15252A-E6 | VGB6 | C57BL/6NTac | |
| 15252A-B2 | VGB6 | C57BL/6NTac | |
