| Gene | Gpr35 |
| Chromosome | 1 |
| Deletion Start | 92,983,493 |
| Deletion End | 92,982,571 |
| Deletion Size | 923 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 92,982,702 | 92,982,722 | CATGCACCAGTGGACAGAGAC |
| TUP | 92,982,723 | 92,982,750 | CCGCATCTATATGACCAACCTGGCTGTG |
| TUR | 92,982,766 | 92,982,788 | AGCACAAATGGCAAGGAGCAGAG |
| TDF | 92,983,085 | 92,983,107 | CCTTCTCACTGCTGGGATTCTAC |
| TDP | 92,983,111 | 92,983,132 | CCGCTGGCCATCGTGGTCTTCT |
| TDR | 92,983,161 | 92,983,179 | ATCAGCGGCTGGCCTTCTC |
PCR Primers
| Primer | Sequence |
| SU | CCAGTGAGCAGAGGTTAGGC |
| SD | TTTGAGGGTCCTCTGGAAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15253A-B6 | VGB6 | C57BL/6NTac | |
| 15253A-D10 | VGB6 | C57BL/6NTac | |
| 15253A-E12 | VGB6 | C57BL/6NTac | |
| 15253A-F10 | VGB6 | C57BL/6NTac | |
| 15253A-G6 | VGB6 | C57BL/6NTac | |
