| Gene | 4933406M09Rik |
| Chromosome | 1 |
| Deletion Start | 134,390,934 |
| Deletion End | 134,388,702 |
| Deletion Size | 2,233 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 134,388,792 | 134,388,813 | CAGCATGTCACCTACACAGTTC |
| TUP | 134,388,825 | 134,388,853 | TCTTACCAGCCAAAGAGTGAGTATGTGGG |
| TUR | 134,388,860 | 134,388,879 | GGCCCTTGCCAAGTCTAATG |
| TDF | 134,390,376 | 134,390,395 | TGTCCCTATCCGCCTCAGTC |
| TDP | 134,390,397 | 134,390,423 | CTCCGTCTTCTACCGAATGGACAGGGA |
| TDR | 134,390,437 | 134,390,455 | TCGCAGGAAAGCATGTGTC |
PCR Primers
| Primer | Sequence |
| SU | TTCCTGCCCTTTCTCAGATG |
| SD | GGAGAATAAATTCCATGATCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15259A-A5 | VGB6 | C57BL/6NTac | |
| 15259A-B2 | VGB6 | C57BL/6NTac | |
| 15259A-H12 | VGB6 | C57BL/6NTac | |
| 15259A-H8 | VGB6 | C57BL/6NTac | |
| 15259A-G6 | VGB6 | C57BL/6NTac | |
| 15259A-F10 | VGB6 | C57BL/6NTac | |
