| Gene | B3galt2 |
| Chromosome | 1 |
| Deletion Start | 143,647,493 |
| Deletion End | 143,646,131 |
| Deletion Size | 1,363 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 143,646,418 | 143,646,441 | CAACTCCAGTAACACCGAGCTATC |
| TUP | 143,646,443 | 143,646,464 | CCACAGGGAGTCACAGGGCTGC |
| TUR | 143,646,473 | 143,646,491 | TGCTGCCATTGGCACTGAG |
| TDF | 143,646,985 | 143,647,006 | TGCACCGAACAGAAACAAAGAC |
| TDP | 143,647,010 | 143,647,037 | AAGTGGTACATGCCACCAGACCTTTACC |
| TDR | 143,647,055 | 143,647,076 | AACCAGTTCCTGAGCAGAAGAC |
PCR Primers
| Primer | Sequence |
| SU | GGTCAAAGGGACAGATGAGC |
| SD | TCATTGCACAATTACAAAGTTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15260A-A11 | VGB6 | C57BL/6NTac | |
| 15260B-A12 | VGB6 | C57BL/6NTac | |
| 15260B-G5 | VGB6 | C57BL/6NTac | |
