| Gene | Kin |
| Chromosome | 2 |
| Deletion Start | 10,092,561 |
| Deletion End | 10,080,634 |
| Deletion Size | 11,928 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 10,080,826 | 10,080,844 | CCTCCAGCTCCACGAGAAG |
| TUP | 10,080,845 | 10,080,868 | TGGTCGCAGACAAGCACTCACTGA |
| TUR | 10,080,876 | 10,080,894 | GTGCTCAGCCTAGCGATTG |
| TDF | 10,091,603 | 10,091,623 | CAAGATCCTGTTGGGAGTCAC |
| TDP | 10,091,626 | 10,091,652 | CATGCATAGTGTGCAGAGCTGGAATGC |
| TDR | 10,091,663 | 10,091,685 | CCGTCCAGATGCAGAGTAAAGTG |
PCR Primers
| Primer | Sequence |
| SU | CCTCCCGATGTGGTCCTAC |
| SD | CTTGTCAGAACCCAGGTCAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15275A-D10 | VGB6 | C57BL/6NTac | |
| 15275A-B11 | VGB6 | C57BL/6NTac | |
| 15275A-B12 | VGB6 | C57BL/6NTac | |
| 15275A-G9 | VGB6 | C57BL/6NTac | |
| 15275A-G12 | VGB6 | C57BL/6NTac | |
