| Gene | Psd4 |
| Chromosome | 2 |
| Deletion Start | 24,407,278 |
| Deletion End | 24,394,129 |
| Deletion Size | 13,150 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 24,394,547 | 24,394,566 | GCATCCCACCACCCTCTAAG |
| TUP | 24,394,569 | 24,394,595 | CCTCCTTGGAGATGAAGGTCTGAGCCC |
| TUR | 24,394,627 | 24,394,644 | AGGCCCACTGCTGTCATC |
| TDF | 24,407,052 | 24,407,074 | GAAGATCAGCTTGGGAAGGAAAC |
| TDP | 24,407,077 | 24,407,098 | ACGGTTCTCAGGAGCCCAGGCC |
| TDR | 24,407,117 | 24,407,136 | GGTGCAGTGATGGGCTTGAG |
PCR Primers
| Primer | Sequence |
| SU | ATCCCAAGATAAAGGCCTGG |
| SD | CCTATGCTTCTGGGACCATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15276A-A8 | VGB6 | C57BL/6NTac | |
| 15276A-C3 | VGB6 | C57BL/6NTac | |
| 15276A-E3 | VGB6 | C57BL/6NTac | |
| 15276A-G4 | VGB6 | C57BL/6NTac | |
| 15276A-E9 | VGB6 | C57BL/6NTac | |
| 15276A-B6 | VGB6 | C57BL/6NTac | |
