| Gene | Exosc2 |
| Chromosome | 2 |
| Deletion Start | 31,680,575 |
| Deletion End | 31,670,743 |
| Deletion Size | 9,833 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 31,670,984 | 31,671,004 | GACACATCGGAGCATCCATAC |
| TUP | 31,671,006 | 31,671,029 | AGTTCCCTCCTTTGCGAGCCTCAC |
| TUR | 31,671,058 | 31,671,079 | GGGAATCAGGAACTGACAAAGG |
| TDF | 31,680,009 | 31,680,031 | GCTCCTGTGTTTCTTCAGTGTTC |
| TDP | 31,680,032 | 31,680,059 | CACTTTGTTTCCCATAACCCAGGCAGGA |
| TDR | 31,680,073 | 31,680,094 | TGGCAGGGACTCATTAAGGTTG |
PCR Primers
| Primer | Sequence |
| SU | TGACATCCAAACACCAAACC |
| SD | TGCCATGTCTTTGTCTTTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 96 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15278A-E12 | VGB6 | C57BL/6NTac | |
| 15278A-F1 | VGB6 | C57BL/6NTac | |
| 15278A-G9 | VGB6 | C57BL/6NTac | |
| 15278A-H9 | VGB6 | C57BL/6NTac | |
