| Gene | Lcn2 |
| Chromosome | 2 |
| Deletion Start | 32,387,714 |
| Deletion End | 32,384,788 |
| Deletion Size | 2,927 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 32,387,478 | 32,387,497 | CTGGGTAGGATGGCTAGGAC |
| TUP | 32,387,447 | 32,387,471 | AGGCCAGTGTCCAGGCTTCTACACA |
| TUR | 32,387,401 | 32,387,421 | TCTGAGCAGCATGAGATGTGG |
| TDF | 32,385,435 | 32,385,456 | CCTCAAGGACGACAACATCATC |
| TDP | 32,385,402 | 32,385,425 | CCCACCGGTAATGGCCAGTTGAGG |
| TDR | 32,385,370 | 32,385,389 | GCTTGGACAGTTCCCACATC |
PCR Primers
| Primer | Sequence |
| SU | TCCAGATCTGAGCTGCTGAC |
| SD | TTGCCCTTCATTGTTATGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15279A-B5 | VGB6 | C57BL/6NTac | |
| 15279A-C10 | VGB6 | C57BL/6NTac | |
| 15279A-G7 | VGB6 | C57BL/6NTac | |
| 15279A-C7 | VGB6 | C57BL/6NTac | |
