Information regarding availability, timelines and delivery of materials can be found at www.komp.org or www.knockoutmouse.org.

15282 Zbtb6 Status: ES cell colonies screened / QC positives

Gene	Zbtb6
Chromosome	2
Deletion Start	37,429,911
Deletion End	37,428,604
Deletion Size	1,308
Genome Build	38

MGI ID	MGI:2442998
NCBI Gene ID	241322
BAC ID	116F24
BAC Library	bMQ
Cassette	ZEN-Ub1
Clones Available	Yes

Sequence Downloads and Strategy Information

Primer Genomic Coordinates on Chromosome 2

Please be aware that these oligonucleotides have been validated only by Taqman assay.

Primer	Start	End	Sequence
TUF	37,429,655	37,429,674	GGCTGGAAGTTGCTGTTGTC
TUP	37,429,634	37,429,654	CTGCTATACTGGAGCACTTGA
TUR	37,429,585	37,429,606	AAGGTAACTGGCAGCAGTCAAG
TDF	37,428,874	37,428,898	TCTTAATCGGCATATTCGAGGACAC
TDP	37,428,843	37,428,872	TGGGTATAAGGCCCTTTCAGTGTACTGTGT
TDR	37,428,792	37,428,815	GTATGTTCAAGTGGTCCTGAAGTG

PCR Primers

Primer	Sequence
SU	TTTGCTAGGAGACCTGTGGG
SD	AGCGATGTGCAACACTATGC
LacInR	TTGACTGTAGCGGCTGATGTTG
LacInf	GGTAAACTGGCTCGGATTAGGG
NeoInR	TACTTTCTCGGCAGGAGCAAGGTG
NeoInF	TTCGGCTATGACTGGGCACAACAG
LacInZRev	GTCTGTCCTAGCTTCCTCACTG
NeoFwd	TCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward Primer	Reverse Primer	Length	WT	HET	KO
SU	LacZRev	bp	-	+	+
TUF	TUR	90 bp	+	+	-
TDF	TDR	107 bp	+	+	-
NeoFwd	SD	bp	-	+	+
LacInf	LacInR	210 bp	-	+	+
NeoInf	NeoInR	282 bp	-	+	+

Available Clones

ES Cell ordering information can be found at the KOMP Repository.

Clone Name	Parental ES Cell	Mouse Strain	Cassette Copy Number
15282A-C4	VGB6	C57BL/6NTac
15282A-D4	VGB6	C57BL/6NTac
15282A-H5	VGB6	C57BL/6NTac