| Gene | Nup35 |
| Chromosome | 2 |
| Deletion Start | 80,658,411 |
| Deletion End | 80,638,896 |
| Deletion Size | 19,516 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 80,639,128 | 80,639,148 | GCCACTGGTCGCTGTAGTAAC |
| TUP | 80,639,152 | 80,639,169 | TTGCCGCTGGGCCACCTA |
| TUR | 80,639,226 | 80,639,244 | GCGAGCCAGGTTGGTTCTG |
| TDF | 80,657,978 | 80,657,998 | CCATTGATTTCCCGAGAGTCC |
| TDP | 80,658,004 | 80,658,027 | TTTGGCACAGGCCATGATACCTGC |
| TDR | 80,658,032 | 80,658,053 | TCTGACAGCTCACGCATACTTC |
PCR Primers
| Primer | Sequence |
| SU | TACTAGTCTTAGAAGCAGACG |
| SD | CTCTCTGAGATTCATCCGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 117 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15287A-C11 | VGB6 | C57BL/6NTac | |
| 15287A-H3 | VGB6 | C57BL/6NTac | |
