| Gene | Scg5 |
| Chromosome | 2 |
| Deletion Start | 113,827,561 |
| Deletion End | 113,791,884 |
| Deletion Size | 35,678 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 113,827,363 | 113,827,381 | GCTCACCAGGCCATGAATC |
| TUP | 113,827,340 | 113,827,362 | TTGTTGGGCCACAGAGCATCGAA |
| TUR | 113,827,315 | 113,827,338 | CTGTCAGTTTGGAAGAGTGCATAC |
| TDF | 113,792,465 | 113,792,486 | TCTGTGACAGTTGCCCTCTATG |
| TDP | 113,792,440 | 113,792,464 | TGTCCCTCATTCCCACCCAAATCCA |
| TDR | 113,792,381 | 113,792,404 | GGTGAATGACTGGGTTTCTGGATG |
PCR Primers
| Primer | Sequence |
| SU | TTTGGGTCTCCCTAACTCCC |
| SD | CCTACAAGCAAGTCAAAGGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 67 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15299A-C1 | VGB6 | C57BL/6NTac | |
| 15299A-E10 | VGB6 | C57BL/6NTac | |
| 15299A-F11 | VGB6 | C57BL/6NTac | |
| 15299A-H5 | VGB6 | C57BL/6NTac | |
